platereader

package

v0.0.0-...-040724e Latest Latest Go to latest Published: Jun 6, 2019 License: BSD-3-Clause, GPL-2.0, BSD-3-Clause, + 1 more Imports: 4 Imported by: 1

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Repository

github.com/antha-lang/antha

Documentation ¶

Overview ¶

Package platereader contains functions for manipulating absorbance readings and platereader data.

Index ¶

func Blankcorrect(blank wtype.Absorbance, sample wtype.Absorbance) (blankcorrected wtype.Absorbance, err error)
func Concentration(pathlengthcorrected wtype.Absorbance, ...) (conc wunit.Concentration)
func EstimatePathLength(plate *wtype.Plate, volume wunit.Volume) (wunit.Length, error)
func PathlengthCorrect(pathlength wunit.Length, reading wtype.Absorbance) (pathlengthcorrected wtype.Absorbance)
func ReadAbsorbance(plate *wtype.Plate, solution *wtype.Liquid, wavelength float64) (abs wtype.Absorbance)
type FilterOption
type ReadMode
type ReadType

Constants ¶

This section is empty.

Variables ¶

This section is empty.

Functions ¶

func Blankcorrect ¶

func Blankcorrect(blank wtype.Absorbance, sample wtype.Absorbance) (blankcorrected wtype.Absorbance, err error)

Limitations of the Beer-Lambert law ¶

The linearity of the Beer-Lambert law is limited by chemical and instrumental factors. Causes of nonlinearity include: deviations in absorptivity coefficients at high concentrations (>0.01M) due to electrostatic interactions between molecules in close proximity scattering of light due to particulates in the sample fluoresecence or phosphorescence of the sample changes in refractive index at high analyte concentration shifts in chemical equilibria as a function of concentration non-monochromatic radiation, deviations can be minimized by using a relatively flat part of the absorption spectrum such as the maximum of an absorption band stray light

func EstimatePathLength ¶

func EstimatePathLength(plate *wtype.Plate, volume wunit.Volume) (wunit.Length, error)

EstimatePathLength estimate the height of liquid of the given volume in the plates welltype - the length of the light path

func PathlengthCorrect ¶

func PathlengthCorrect(pathlength wunit.Length, reading wtype.Absorbance) (pathlengthcorrected wtype.Absorbance)

func ReadAbsorbance ¶

func ReadAbsorbance(plate *wtype.Plate, solution *wtype.Liquid, wavelength float64) (abs wtype.Absorbance)

Types ¶

type FilterOption int

FilterOption defines the type of platereader data which will be returned by the ReadingsAsAverage method on a dataset.

const (
	// TIME will return readings matching a specified time
	TIME FilterOption = iota
	// EMWAVELENGTH will return readings matching a specified Emmission wavelength
	EMWAVELENGTH
	// EXWAVELENGTH will return readings matching a specified Excitation wavelength
	EXWAVELENGTH
)

type ReadMode ¶

type ReadMode string // or could just make this a string

ReadMode defines the mode of plate reader reading.

const (
	// Absorbance defines the reading to be an absorbance reading.
	Absorbance ReadMode = "Absorbance"
	// Fluorescence defines the reading to be a fluorescence reading.
	Fluorescence ReadMode = "Fluorescence"
)

type ReadType ¶

type ReadType string // or could just make this a string

ReadType defines the type of plate reader reading.

const (
	// Endpoint defines the reading to be an endpoint.
	Endpoint ReadType = "Endpoint"
)

Source Files ¶

View all Source files

Directories ¶

Path	Synopsis
dataset interface platereaderparse.go Part of the Antha language Copyright (C) 2015 The Antha authors.	interface platereaderparse.go Part of the Antha language Copyright (C) 2015 The Antha authors.
parse

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